Rnase inhixitor 2500unit -MO5441(سیناکلون)

Ribonuclease Inhibitor فعالیت RNases A,B and C را بوسیله اتصال به آنها به صورت غیر رقابتی مهار میکند.

مشخصات کیت:

• این آنزیم برروی RNases 1 ,T1 ,T2 ,U1, H ,U2 ,CL3 اثر مهار ندارد.

کاربرد کیت:

• Ribonuclease Inhibitor(RNase Free)
  For Research Use Only
  Catalogue No. : MO5441 Quantity: 2500U
  Concentration: 40U/µl Store at: -20°C
  Description:
  Ribonuclease Inhibitor RNase- Free inhibits the activity of RNase A,B,C by binding them in a noncompetitive mode
  at a 1:1 ratio. It dose not inhibit RNase 1, T1, T2, H, U1, U2, CL3 and other enzymes.
  Features:
   Performs under a wide range reaction conditions.
   Protects RNA from degradation at temperature up to 55°C.
   Increase the time RNA can safety stored.
  Assay Conditions:
  100mM Tris-HCL (PH 7.5), 1.2mM EDTA, 0.1mg/ml BSA, 100ng/ml RNase , 0.1mg/ml E.coli]
  3H[-RNA,50mg/ml yeast
  RNA and 8mM DTT.
  Inactivation: DTT at final concentration of 5mM.
  Unit Definition:
  1u is defined as the amount of ribonuclease inhibitor that inhibits the activity of 5ng Ribonuclease A by 50%.
  Application:
   Applied in procedures where RNase contamination constitutes a problem:
  – In vitro transcription
  – In vitro translation
  – cDNA synthesis
  – isolation of mammalian cell fractions that contain mRNA-protein complex.
  – Separation and identification of specific ribonuclease activities.
  Transcriptase or Taq DNA polymerase.
   Increase the time RNA can be safely stored.
  Storage Buffer:
  20mM HEPES-KOH (pH 7.6), 50mM KCl, 5mMDTT, and 50% glycerol.
  Quality Control:
  The absence of endodeoxyribonucleases, exodeoxyribonucleases,phosphatase and ribonucleases confirmed by
  appropriate quality tests. Functionally tested in RNA and cDNA synthesis.